PCNA anticorps (C-Term)

N° du produit ABIN7455223

L’anticorps Lapin Polyclonal anti-PCNA a été validé pour WB, IP, IF (p) et IHC (fp). Il convient pour détecter PCNA dans des échantillons de Humain et Souris.

Aperçu rapide pour PCNA anticorps (C-Term) (ABIN7455223)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

Détail du produit anti-PCNA anticorps

Épitope: C-Term

Fonction: Rabbit anti-PCNA Antibody, Affinity Purified

Homologie: Rat,Bovine

Purification: Affinity Purified

Immunogène: between AA 225 and C-term

Isotype: IgG

Information d'application

Indications d'application:

IHC-IF: 1:1,000 - 1:5,000

IHC: 1:2,000 to 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

IP: 2 - 10 μg/mg lysate

WB: 1:5,000 - 1:20,000

Restrictions: For Research Use only

Stockage

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Date de péremption: 12 months

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: Background: PCNA (Proliferating Cell Nuclear Antigen) was first identified as a factor expressed in the nuclei of replicating cells. PCNA plays a role in both DNA synthesis and repair. PCNA functions as a processivity factor for DNA polymerase by acting as a DNA clamp that tethers the polymerase catalytic units to the DNA template. The structure of PCNA is that of a trimeric ring that encircles and slides along replicating DNA. PCNA interacts with and is regulated by several proteins involved in cell cycle control and check point processes.

ID gène: 5111

NCBI Accession: NP_872590

UniProt: P12004

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy